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Sequence clusters were created from Build of UniGene April 20, and refined by analysis and comparison with a of other publicly available databases including the Washington University EST trace repository and the University of California, Santa Cruz golden-path human genome database April release. In addition, ESTs were analyzed for untrimmed low-quality sequence information, correct orientation, cuat priming, false clustering, alternative splicing and alternative polyadenylation.

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In addition, ESTs were analyzed for untrimmed low-quality sequence information, correct orientation, false priming, false clustering, alternative splicing and alternative polyadenylation. The set includes over 1, unique oligonucleotide features covering more than 39, transcript variants, which in turn represent greater than 33, of the best characterized human genes.

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